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KMID : 0380020060210020079
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 2 p.79 ~ p.84
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Enhancement of L-Lysine Productivity by Strain Improvement and Optimization of Fermentation Conditions in Corynebacterium glutamicum
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Seo Jin-Mi
Hyun Hyung-Hwan
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Abstract
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In order to minimize the reduction of lysine productivity by accumulation of lysine and byproducts in the end of fed-batch
fermentations, a salt-tolerant mutant C14-49-3-15-7-3-20, which could grow at high concentrations of NaCl was isolated
through mutagenesis from the Corynebacterium glutamicum mother strain I. In the evaluation of L-lysine productivity by
fed-batch fermentations using a 5 L jar fermenter, the salt-tolerant mutant strain C14-49-3-15-7-3-20 produced 130.6 g/L of
L-lysine with a 48.6% of yield. The mother strain I produced L-lysine concentration only 104.9 g/L with a yield 41.8%,
implying the improvement of L-lysine productivity by introduction of salt-tolerance character.
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KEYWORD
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L-lysine, Corynebacterium glutamicum, fed-batch fermentation, salt-tolerance
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